From: Processing of collagen based biomaterials and the resulting materials properties
Tissue | Species | Form [2D; 3D] | Freeze/thaw cycles | Washing cycles | Surfactant | Acid/base | Enzyme treatment | Complexing agents | Mechanical treatment | Sterilisation /decontamin. | Time (h) | Refs. |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Water, buffer, salts | SDS, Triton X, others | Organic, inorganic | DNAse, protease | CHAPS, EDTA, etc | ||||||||
SIS | Porcine | 2D | + | 125 | 13 | − | − | − | (−) | − | 138 | [492] |
Pericardium | Bovine | 2D | − | 1 | − | 3 | − | − | − | Gamma | 4 | [46] |
Tendon | Diverse | 3D |  |  |  |  |  |  |  |  | 5–48 (96) | [493] |
Dermis | Human | 2D | + | 19 | − | − | 3 | 47 | − | Gamma | 69 | [494] |
Dermis | Porcine | 2D | + | 18*** | 22** | − | 6 | (+) | (+) | PAA; H2O2* | 49 | [495] |
Dermis | Porcine | Dispers | + | 4 | 2 | 8 | − | − | + | − | 13 | [496] |
Dermis | Porcine | Dispers | + | 35 | − | 39 | − | − | + | H2O2 | 74 | Own trials, unpublished |