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Table 7 Frame of wet technologies to decellularize diverse tissues

From: Processing of collagen based biomaterials and the resulting materials properties

Tissue Species Form [2D; 3D] Freeze/thaw cycles Washing cycles Surfactant Acid/base Enzyme treatment Complexing agents Mechanical treatment Sterilisation /decontamin. Time (h) Refs.
Water, buffer, salts SDS, Triton X, others Organic, inorganic DNAse, protease CHAPS, EDTA, etc
SIS Porcine 2D + 125 13 (−) 138 [492]
Pericardium Bovine 2D 1 3 Gamma 4 [46]
Tendon Diverse 3D          5–48 (96) [493]
Dermis Human 2D + 19 3 47 Gamma 69 [494]
Dermis Porcine 2D + 18*** 22** 6 (+) (+) PAA; H2O2* 49 [495]
Dermis Porcine Dispers + 4 2 8 + 13 [496]
Dermis Porcine Dispers + 35 39 + H2O2 74 Own trials, unpublished
  1. The numbers reflect hours of treatment
  2. as, antiseptic processing; ab, addition of antibiotics
  3. * Additionally other sterilization techniques were investigated
  4. ** Complexing agents and surfactant are applied together
  5. *** Washing comprised 12 h ethanol treatment