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Table 5 Different kinetics have to be considered which influence the processing times during tissue decellularization, purification and processing

From: Processing of collagen based biomaterials and the resulting materials properties

Time scale

T; T opt (°C)

Reaction rate

Remark

Refs.

pH 3

pH 7

pH 12

Denaturation

> TD

Seconds

Seconds to hours

Seconds

Depending on the degree of structural organization, crosslinking degree, solvent concentration and additives

[181, 480,481,482,483]

Renaturation

0 to ~ 20; 4 to 10

Hours to months

Hours to months

Hours to months

Dependent on the sequence, molecular length and covalent crosslinks

[152, 481, 484, 485]

Reassembly/fibrillation

10 to 38; 20 to 35

Minutes to hours

Only possible in neutral conditions

[186, 190, 191, 486,487,488]

Disassembly

< 37; < 5

Seconds

Minutes to hours

Seconds

At pH deviating from the IEP a high swelling is observed which can be assumed as disassembly [343]

[189, 191, 487]

Crosslinking reaction

 

Seconds to hours

Seconds to hours

Seconds to hours

Highly dependent on the kind of reaction

[192, 248, 420, 421, 489,490,491]

Diffusion

 

Hours

Minutes to hours

Hours

See special section