Table 2 PCR protocol used in the experiments
From: Performance evaluation of optimal real-time polymerase chain reaction achieved with reduced voltage
PCR process | Temperature (°C) | Time duration |
|---|---|---|
1 cycle | 95 | 3 min |
40 cycles | 95 | 10 s |
60 | 30 s | |
72 | 30 s | |
1 cycle | 95 | 10 s |
50 | 30 s |