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Fig. 6 | BioMedical Engineering OnLine

Fig. 6

From: Wound healing of human embryonic stem cell-derived retinal pigment epithelial cells is affected by maturation stage

Fig. 6

Percentage of cells with spontaneous [Ca2+]i increases (%RC) in wounded hESC-RPEs at different areas related to the wound location. A bright field image of 9-day-cultured, wounded hESC-RPEs, visualized immediately after wounding (A). A fluorescence image of the same sample as in A loaded with Ca2+-sensitive indicator Fluo-4 AM (B). The wound area (“wound”) and adjacent areas (“Area 1”, “Area 2”, “Area 3”) are marked with white lines. Analyzed percentage of responsive cells (%RC) in non-wounded control (ns = 8), and area 1 (ns = 8), area 2 (ns = 8) and area 3 (ns = 8) (C). Statistical significance: p < 0.05 is marked with Asterisk. A bright field image of 9-day-cultured then wounded hESC-RPEs, visualized 7 days after wounding (D). A fluorescence image of the same sample as in D loaded with Ca2+-sensitive indicator fluo-4 AM (E). The wound area and adjacent areas are marked with white lines. The %RC in non-wounded control (ns = 8), wounded area (ns = 8) and area 1 (ns = 8), area 2 (ns = 8), and area 3 (ns = 6) (F). A bright field image of 28-day-cultured and wounded hESC-RPEs, visualized immediately after wounding (G). A fluorescence image of the same sample as in G loaded with Ca2+-sensitive indicator Fluo-4 AM (H). The wound area and adjacent areas are marked with white lines. The %RC in non-wounded control (ns = 8) and area 1 (ns = 8), area 2 (ns = 8), and area 3 (ns = 8) (I). A bright field image of 28-day-cultured then wounded hESC-RPEs, visualized 7 days after wounding (J). A fluorescence image of the same sample as in J loaded with Ca2+-sensitive indicator Fluo-4 AM (K). The wound area and adjacent areas are marked with white lines. The %RC in non-wounded control (ns = 4), wounded area (ns = 4) and area 1 (ns = 4), area 2 (ns = 4), and area 3 (ns = 4) (L). The presented data are combined from Regea08/017 and Regea08/023 hESC-RPEs

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