Fig. 2

Morphology and polarity of hESC-RPE cultures. Localization of tight junction protein ZO-1 (red) and Ki67-positive proliferative cells (green) in control and wounded Regea08/017 and Regea08/023 hESC-RPEs. Nuclei were counterstained with DAPI (blue). Non-wounded area of Regea08/017 hESC-RPEs cultured for 35 days (A–C). Regea08/017 hESC-RPEs wounded on day 28 of culture and allowed to heal for 7 days, 35 days of culture in total (D–F). Non-wounded area of Regea08/023 hESC-RPEs cultured for 35 days (G–I). Regea08/023 hESC-RPEs wounded on day 28 of culture and allowed to heal for 7 days, 35 days of culture in total (J–L). On the left row are the Z-stack from the apical side, in the middle from the nuclear level, and on the right row from the basal side of the culture. The cross-hairs of Z-plane indicators are intensified for clarity. Scale bar 10 μm