Fig. 7
NGF and GDNF protein expression of Schwann cells seeding on different titanium substrate at 48 h (mean ± SD, n = 6). a Whole cell extracts were analyzed by Western blotting to determine expression levels of NGF and GDNF following seeding on different substrate with minocycline. b, c NGF and GNDF protein expression was quantified by measuring gray levels by Image J software. β-Actin was used to normalize target proteins. Asterisk (*) indicate significant difference values compared with pure titanium group. Hash (#) indicate significant difference values compared with TNT group (p < 0.05)