Fig. 1
From: Multi-tissue and multi-scale approach for nuclei segmentation in H&E stained images
Processing for obtaining the initial threshold for different tissues (rows), showing images with a high variation of cells number, size and color. Starting from the RGB image, the PWMCURVE is estimated from its grayscale histogram. Then, candidate thresholds are evaluated as inflection points of the curve (red dotted lines). The median area of detected objects using candidate thresholds is calculated and the initial threshold is determined as the one with the highest median area. In the last column, the application of the initial threshold on the RGB image is shown