Numerical study of gene electrotransfer efficiency based on electroporation volume and electrophoretic movement of plasmid DNA

Table 2 Model-based prediction of gene electrotransfer efficiency resulting from the pulse combination 1000 V/cm (100 μs) + 140 V/cm (400 ms)

	Plate (LTR diameter = 10 μm)	Plate (LTR diameter = 20 μm)	Finger	Needle
Volume of reversible electroporation—dermis (mm³)	2.61	3.61	6.18	10.19
Volume of reversible electroporation—all layers (mm³)	16.36	21.63	216.40	330.72
pCMV-luc (6233 bp)
Number of charged particles inside the volume of reversible electroporation—dermis	307	422	1024	937
Number of charged particles inside the volume of reversible electroporation—all layers	310	422	1205	994
INVAC-1 (7120 bp)
Number of charged particles inside the volume of reversible electroporation—dermis	305	387	941	870
Number of charged particles inside the volume of reversible electroporation—all layers	305	389	1122	931

The measure of gene electrotransfer efficiency is the number of charged particles representing plasmid DNA inside the volume of reversible electroporation at the end of the pulse delivery. From all particles released from the surface of the plasmid volume, 5000 were selected randomly for evaluation. Increasing the number of evaluated particles did not affect the relative gene electrotransfer efficiencies

ISSN: 1475-925X