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Table 2 Model-based prediction of gene electrotransfer efficiency resulting from the pulse combination 1000 V/cm (100 μs) + 140 V/cm (400 ms)

From: Numerical study of gene electrotransfer efficiency based on electroporation volume and electrophoretic movement of plasmid DNA

  Plate (LTR diameter = 10 μm) Plate (LTR diameter = 20 μm) Finger Needle
Volume of reversible electroporation—dermis (mm3) 2.61 3.61 6.18 10.19
Volume of reversible electroporation—all layers (mm3) 16.36 21.63 216.40 330.72
pCMV-luc (6233 bp)
 Number of charged particles inside the volume of reversible electroporation—dermis 307 422 1024 937
 Number of charged particles inside the volume of reversible electroporation—all layers 310 422 1205 994
INVAC-1 (7120 bp)
 Number of charged particles inside the volume of reversible electroporation—dermis 305 387 941 870
 Number of charged particles inside the volume of reversible electroporation—all layers 305 389 1122 931
  1. The measure of gene electrotransfer efficiency is the number of charged particles representing plasmid DNA inside the volume of reversible electroporation at the end of the pulse delivery. From all particles released from the surface of the plasmid volume, 5000 were selected randomly for evaluation. Increasing the number of evaluated particles did not affect the relative gene electrotransfer efficiencies