Fig. 4

Expression of special cardiomyocyte genes by qRT-PCR in 2D and 3D groups (A). Significant increase of NKX2.5 and GATA4 and also significant decrease of β-MHC is observe in this figure (*P < 0.05; Data presented are mean ± SD. n = 3). Detection of Nkx2.5 marker by immunocytochemistry (B) in 3D (c1, c2 and c3) and 2D (b1, b2 and b3) groups. Human heart stained as the positive control (a1, a2 and a3). Cell nuclei (blue) and Nkx2.5 protein in the nucleus (green) stained by Hoechst. Detection of cardiac Troponin I marker by immunocytochemistry (C) in 3D (c1, c2 and c3) and 2D (b1, b2 and b3) groups. Human heart stained as the positive control (a1, a2 and a3). Cell nuclei (blue) and cardiac Troponin I protein in the nucleus (green) stained by Hoechst