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Fig. 4 | BioMedical Engineering OnLine

Fig. 4

From: Simulation of developing human neuronal cell networks

Fig. 4

a Proportion of GABAergic cells in neuronal population analyzed at different measurement time points (MTP). Standard deviations for calculated GABA-positive cell percentages in measurement time points 2, 3, 4, and 32 days in vitro (DIV) are 17, 9, 13 and 10 %, respectively. b Representative image of GABA-positive cells. c Representative image of neuronal network double-labeled with GABA. d Cells expressing calcium binding protein Calretinin form a subpopulation of GABAergic cells. e Expression of GABA and GABA synthesizing enzyme glutamate decarboxylase labeled with GAD67 define GABAergic neurons. Nuclei (blue) are stained with DAPI. The used magnification for b and c is ×10 and for d and e ×20

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