Fig. 2

Comparison of spike trains and ISI histogram of both the experimental and simulated data. a The upper row shows snippets of example spike trains of the measured hESC-NNs at five electrodes of dataset #9 (electrode number on the y axis). The middle row shows the raw voltage traces of channel 63. The lower row represents the resulting spike trains of five simulated neurons. Each row shows measurement time point 1, 3 and 5, respectively. The length of the detected bursts is indicated as bars on top of the spikes. b The upper row shows the ISI histogram of one channel/ neuron. On the left, an ISI histogram of channel 63 at measurement time point 5 (22 DIV). On the right, an ISI histogram of a simulated neuron at vMTP 5. The lower row shows the population ISI histogram of dataset #9 at MTP 5 on the left and the population ISI histogram of the neuronal network at vMTP 5. Note that we compare the ISIs of 20 active MEA electrodes where the exact number of recorded neurons is unknown with ISIs of 1000 simulated neurons. Thus, the absolute number of spikes cannot be compared and the main information is in the distribution of the histogram