Fig. 7From: Two-hierarchical nonnegative matrix factorization distinguishing the fluorescent targets from autofluorescence for fluorescence imagingUnmixing results for BALB/c mouse experiment 2. a-1–a-4 Raw fluorescence images acquired at the 525, 542, 579 and 624 nm emission filters after subcutaneous injection of AF488 and AF555 dyes into BALB/c mouse. The AF488 in the first three images is excited at 474 nm and AF555 in the last two images at 500 nm. The unmixed AF488, AF555, and AF obtained with: b-1–b-3 MCR-ALS, c-1–c-3 NMF\({{l}_{1}}\), d-1–d-3 sNMF, e-1–e-3 thNMFBack to article page