Fig. 6

Unmixing results for BALB/c mouse experiment 1. a-1–a-5. Raw fluorescence images acquired at the 525, 542, 579, 624 and 716 nm emission filters after subcutaneous injection of AF488 and AF594 dyes into BALB/c mouse. The AF488 in the first three images is excited at 474 nm, the AF594 in the last two images is excited at 565 nm. The unmixed AF488, AF594, and AF obtained with: b-1–b-3 MCR-ALS, c-1–c-3 NMF\({{l}_{1}}\), d-1–d-3 NMFsc, e-1–e-3 sNMF, f-1–f-3 thNMF