A comparative study of mechanical strain, icariin and combination stimulations on improving osteoinductive potential via NF-kappaB activation in osteoblast-like cells

Table 1 The real-time RT-PCR oligonucleotide primers

Gene	Primer	Sequence (5′–3′)	PCR product (bp)
ALP	Forward	TGACCTTCTCTCCTCCATCC	183
ALP	Reverse	CTTCCTGGGAGTCTCATCCT	183
OCN	Forward	TGCTTGTGACGAGCTATCAG	148
OCN	Reverse	GAGGACAGGGAGGATCAAGT	148
Col I	Forward	GAGCGGAGAGTACTGGATCG	142
Col I	Reverse	GTTCGGGCTGATGTACCAGT	142
BMP-2	Forward	CCCCAAGACACAGTTCCCTA	169
BMP-2	Reverse	GAGACCGCAGTCCGTCTAAG	169
BMP-4	Forward	TGAGCCTTTCCAGCAAGTTT	180
BMP-4	Reverse	CTTCCCGGTCTCAGGTATCA	180
β-Actin	Forward	AGAGGGAAATCGTGCGTGAC	138
β-Actin	Reverse	CAATAGTGATGACCTGGCCGT	138

ISSN: 1475-925X