Figure 7

Effect of mechanical strain, icariin, the combination of mechanical strain and icariin stimulation on NF-κB signaling pathway. After stimulation of mechanical strain, icariin, the combination of mechanical strain and icariin on MC3T3-E1 cells of 1 h/day for 3 days, the total protein were then prepared and western blotted for IκB-α, p-IκB-α, p65, P-p65 using specific IκB-α, p-IκB-α, p65, P-p65 antibodies (a). The β-actin protein was used as internal control. Optical density analysis (b) was obtained with the Image-Pro Plus version 6.0. Data represent mean ± SD values from three independent experiments. *P < 0.05, **P < 0.01 (n = 4) compared with control group, ^# P < 0.05, ^## P < 0.01 compared with mechanical strain or icariin stimulation.