Figure 3

Effect of mechanical strain, icariin, the combination of mechanical strain and icariin stimulation on ALP activity and mRNA expression. After stimulation of mechanical strain, icariin, the combination of mechanical strain and icariin on MC3T3-E1 cells of 1 h/day for 3 days, the total protein and mRNA of MC3T3-E1 cells were extracted. The ALP activity was analyzed by ALP vitality assay detection kit (a) and the mRNA expression of ALP was analyzed by real-time RT-PCR (b). Data represent mean ± SD values from three independent experiments. *P < 0.05, **P < 0.01 (n = 4) compared with control group, ^# P < 0.05, ^## P < 0.01 compared with mechanical strain or icariin stimulation.