Figure 6

Cell segmentation step. a Selected object in RGB, b segmented nucleus, c convex hull of nucleus region, d radii line with centre at the centroid of convex hull, radius equal to major axis length, angle between radii line is 0.3 rad, blue point is the candidate point, e applied direct least square for ellipse fitting to the candidate point, f segmented result: the red contour is the nucleus region, the green contour is the cell region, g–i and j–l are the repeated step of d–f with angle between radii line of 0.2 and 0.1 rad respectively.