Figure 1

A schematic figure of the experimental setup consisting of 1) LOC; gas-tight lab-on-a-chip, with an integrated micropipette, combined with 2) optical tweezers consisting of an (NIRL) NIR-laser, (M) mirrors, (DM) dichroic mirror and an (IRBF) IR blocking filter (to block the IR laser to the CCD), 3) (UVVS) UV-Vis absorption spectrometer including an integrated (OF) optical fiber, 4) CCD camera of the microscope (to monitor the trapping dynamics and the pipette cell patch), 5) (OSU) oxygen sensor unit connected to (OFP) optical Foxy Probe inserted into inlet of the LOC), 6) patch clamp set-up consisting of (MDD) micromanipulator connected to (HS) patch clamp head stage with (PH) pipette holder for insertion of the (PCP) patch clamp pipette through and adapter into the chip, (RE) recording electrode connected to (PCA) patch clamp amplifier through the (HS) and the reference electrode connected between an inlet of the LOC and (HS).