Four steps of the sequential analysis (A-D) of the electroporation process for two electrodes. The optimum parameters as determined by the genetic algorithm were: d = 56 mm, z = 40 mm, ϕ = 90°, U = 1550 V. The electric field distribution is shown for a plane perpendicular to electrode insertion 2 cm deep in muscle tissue. The black contour represents the muscle area that has been reversibly electroporated, i.e. area where the conductivity values have changed according to (3). Due to ratio problems, the contour for irreversibly electroporated muscle tissue is left out of the figure; however it is approximately the same as the dark red coloration surrounding the electrodes in sequence D.