Skip to main content
Figure 5 | BioMedical Engineering OnLine

Figure 5

From: A probabilistic cell model in background corrected image sequences for single cell analysis

Figure 5

Estimated background, corrected images, and normalized histograms of top left part of estimated background based on applying [23]and the point-wise method B ^ 0 for phenotype 1. The presence of quasi-stationary cells in the mid-left of the image sequence causes [23] to interpret the cells as belonging to the background, leading to a cell-background contrast loss in such locations after background subtraction, as we can see in the subtracted image, whereas our proposed point-wise method has quasi-stationary cells only barely appearing in the estimated background. There is a significant number of pixels with intensities higher than the background in the top normalized histogram, showing that some cell boundary pixels are interpreted as background by [23] increasing the misdetection rate.

Back to article page