Skip to main content
Figure 2 | BioMedical Engineering OnLine

Figure 2

From: Non-viral eNOS gene delivery and transfection with stents for the treatment of restenosis

Figure 2

Local Arterial eNOS Transgene Expression and NO Production. (A) Quantitative PCR analysis of endothelial nitric oxide synthase (eNOS) mRNA expression in the rabbit iliac artery after 1 day and 5 days post-implantation of control and eNOS plasmid DNA-containing lipopolyplex (LPP) stents. (B) Transfected eNOS protein levels measured by ELISA were normalized to total protein content in the tissue samples. (C) Nitrite levels as measured by Griess reaction and normalized to total protein content in the tissue samples. The samples (n = 5 per group) represent bare-metal stents (bare metal), poly(D,L-lactide-co-glycolide) (PLGA)//type B gelatin-coated stent (gelatin only), naked eNOS plasmid embedded in PLGA/gelatin-coated stent (gelatin eNOS), eNOS LPP embedded in PLGA/gelatin-coated stent (gelatin LPP). * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 as assessed by ANOVA.

Back to article page