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Figure 2 | BioMedical Engineering OnLine

Figure 2

From: Monitoring and analysis of dynamic growth of human embryonic stem cells: comparison of automated instrumentation and conventional culturing methods

Figure 2

Characterization of hESC colonies cultured in conventional hESC medium: (a) A representative growth graph of an HS293 colony cultured using the automated culture platform for 7 d after passaging. The growth rate of the colony was most rapid from day 5 onwards. An HS293 colony at day 1 (b), day 3 (c), and day 7 (e) after passaging, monitored using the automated system. (d) FACS analysis of HS237 colonies at day 7 after passaging. The red column represents the negative control sample and the white column represents SSEA4-positive cells (81%). An HS293 colony monitored with the automated system was immunohistochemically characterized (f) using an antibody against an undifferentiated hESC marker, Nanog (green), and a differentiated hESC marker, SSEA1 (red). Scale bar 200 μm (b,c,e), 100 μm (f).

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