Skip to main content

Table 2 MPE laser table.

From: Multi-photon excitation microscopy

Laser Material Company; Model Wavelength/nm Pulse Length Repetition Rate Power
Ti:Sapphire Coherent; Mira 700–980 <200 fs 76 MHz 0.7 W 1.3 W
  Spectra Physics; Tsunami 700–1000 <100 fs (or 2ps as option) 80 MHz 0.8 W 1.4 W
  Coherent; Chameleon – XR 705–980 <140 fs 90 MHz 1.7 W
  Spectra Physics; Mai Tai 710–990 120 fs 80 MHz 1.5 W
  Time Bandwidth; Pallas 780–860 <100 fs 75 MHz 500 mW
  Time Bandwidth; Tiger 780–860 <100 fs 100 MHz 400 mW
  Femtosource 750–850 <12 fs 75 MHz 400 mW 600 mW
Nd:YLF MicroLase/Coherent Scotland; BioLite 1047 200 fs 120 MHz 500 mW
Nd:Glass Time Bandwidth; GLX200 1058 <250 fs 100 MHz >400 mW
Ytterbium Amplitude Systems 1030 <200 fs 50 MHz 1 W
Cr:LiSAF Highqlasers 850 snm 100 fs 50 MHz >1--mW
OPO Coherent and Spectra Physics 350–1200 100 fs   ~200 mW
  1. Commercial laser sources available for MPE microscopy and spectroscopy are reported along with main parameters. The pulse length given is the average pulse length through over the full tuning range of the system. Power is the average power at the peak of the tuning curve, typically 800 nm, or at the operating wavelength. Both Coherent Mira and Spectra Physics Tsunami systems can be pumped with a 5,8 or 10 W green laser source. When two powers are given these are for 5 W and 10 W pumped. Higher pump power may be needed for full tuning range. Between 925 and 960 nm Ti:Sapphire lasers need to be purged to ensure that no water vapour is present within the laser cavity. Computer tunable systems are sealed to prevent this complication and are endowed of a pointing stability system that reduces the need of new alignment following tuning across a wide range of wavelengths. The authors acknowledge John Girkin for providing more complete data.