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Table 1 2PE excitation parameters.

From: Multi-photon excitation microscopy

FLUOROPHORES λ (nm) ηδ2 δ2
Extrinsic fluorophores    
Bis-MSB 691/700 6.0 ± 1.8 6.3 ± 1.8
Bodipy 920 17 ± 4.9 -
Calcium Green 740–990 - ~80
Calcofluor 780/820 - -
Cascade blue 750–800 2.1 ± 0.6 ~3
Coumarin 307 776, 700–800 19 ± 5.5 ~20
CY2 780/800 - -
CY3 780 - -
CY5 780/820 - -
DAPI (free) 700/720 0.16 ± 0.05 ~3.5 *
Dansyl 700 1 -
Dansyl Hydrazine 700 0.72 ± 0.2 -
Dil 700 95 ± 28 -
Filipin 720 - -
FITC 740–820 - ~25–38 *
Fluorescein (pH ~11) 780 - 38 ± 9.7
Fura-2 (free) 700 11 -
Fura-2 (high Ca) 700 12 -
Hoechst 780/820 - -
Indo-1 (free) 700 4.5 ± 1.3 12 ± 4
Indo-1 (high Ca) 590/700 1.2 ± 0.4 2.1 ± 0.6
Lucifer Yellow 840–860 0.95 ± 0.3 ~2
Nile Red 810 - -
Oregon Green Bapta 1 800 - -
Rhodamine B 840 - 210 ± 55
Rhodamine 123 780–860 - -
Syto 13 810 - -
Texas red 780 - -
Triple probe (Dapi, FITC, and Rhodamine) 720/740 - -
TRITC (rhodamine) 800–840 - -