Table 1 2PE excitation parameters.
FLUOROPHORES | λ (nm) | ηδ2 | δ2 |
|---|---|---|---|
Extrinsic fluorophores | Â | Â | Â |
Bis-MSB | 691/700 | 6.0 ± 1.8 | 6.3 ± 1.8 |
Bodipy | 920 | 17 ± 4.9 | - |
Calcium Green | 740–990 | - | ~80 |
Calcofluor | 780/820 | - | - |
Cascade blue | 750–800 | 2.1 ± 0.6 | ~3 |
Coumarin 307 | 776, 700–800 | 19 ± 5.5 | ~20 |
CY2 | 780/800 | - | - |
CY3 | 780 | - | - |
CY5 | 780/820 | - | - |
DAPI (free) | 700/720 | 0.16 ± 0.05 | ~3.5 * |
Dansyl | 700 | 1 | - |
Dansyl Hydrazine | 700 | 0.72 ± 0.2 | - |
Dil | 700 | 95 ± 28 | - |
Filipin | 720 | - | - |
FITC | 740–820 | - | ~25–38 * |
Fluorescein (pH ~11) | 780 | - | 38 ± 9.7 |
Fura-2 (free) | 700 | 11 | - |
Fura-2 (high Ca) | 700 | 12 | - |
Hoechst | 780/820 | - | - |
Indo-1 (free) | 700 | 4.5 ± 1.3 | 12 ± 4 |
Indo-1 (high Ca) | 590/700 | 1.2 ± 0.4 | 2.1 ± 0.6 |
Lucifer Yellow | 840–860 | 0.95 ± 0.3 | ~2 |
Nile Red | 810 | - | - |
Oregon Green Bapta 1 | 800 | - | - |
Rhodamine B | 840 | - | 210 ± 55 |
Rhodamine 123 | 780–860 | - | - |
Syto 13 | 810 | - | - |
Texas red | 780 | - | - |
Triple probe (Dapi, FITC, and Rhodamine) | 720/740 | - | - |
TRITC (rhodamine) | 800–840 | - | - |