Morphology and expression of Pdx1 and Nanog, and growth activity. A. Morphology of 12 clones after 30-day culture (passage 7). Scale bars = 200 μm. B. Pdx1 gene expression of 6 candidate clones. These 6 clones were selected based on their ability to grow in culture for over 30 days. Pancreatic stem cells from pancreatic tissue (data not shown) were used as a positive control. C. Nanog (GFP) expression of D#22 and iPS cells. The iPS cells but not D#22 cells expressed GFP. D. Morphology of D#22 cells at several passages. Scale bars = 200 μm. E. Growth activity of D#22 cells at passages 10 and 50.