Expression of myogenic co-factors and mesenchymal markers in stretched hASCs (12%, 1Hz). (A) Myocd, (B) MKL1 and (C) MKL2 gene expression were analyzed by RT-PCR after strain for 72 (n = 5) or 96 hours (n = 6). Each bar represents means ± SEM. (D) Representative dot blot and (E) percentage quantification of CD29/CD90 positive mesenchymal markers in static and stretched hASCs for 96 hours analyzed by flow cytometer (n = 4). Bar represents means ± SEM.