Expression of Smooth Muscle Cell markers in hASCs that underwent stretching (12%, 1Hz). (A) Calponin and (B) SM-MHC gene expression were analyzed by RT-PCR after strain for 72 (n = 5) or 96 hours (n = 6). Each bar represents means ± SEM. (C) ACTA2 and (D) TAGLN gene expression were evaluated by qRT-PCR after stretching for 72 (n = 5) or 96 hours (n = 6). Data are represented as means ± SEM. All data are represented as fold induction relative to Static hASCs at 72 hours.