Pressure loading device. The pressure loading device consisted of a cylinder, a piston, and a pendulum impactor equipped with a weight (A). A pressure chamber equipped with a pressure transducer is connected to the cylinder (B). The compartment connected between the cylinder and the pressure chamber is filled with water. The lower part of the pressure chamber, which is divided by a silicone membrane, is filled with culture medium. Positive pressure or negative pressure is generated by striking the piston with the pendulum from the right or the left (C). The peak pressure and time of an initial pulse in the generated pressure wave is referred to as the pressure amplitude and pressure duration (D).