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Figure 1 | BioMedical Engineering OnLine

Figure 1

From: Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy

Figure 1

The schematic diagram of infrared spectrum of the contents of lipids and proteins in cells. Cells were adjusted to 2 × 106/ml and washed twice with 0.9% NaCl in 1000 RPM centrifugation for 6 min. The supernatant was removed by centrifugation. The cells were transferred to the CaF2 crystals at 37°C and left to stand for about 10 min. The water in the cell suspension was evaporated, until the formation of 2 ~ 3 mm film in the window. The crystals were fixed in the sample holder and covered with another CaF2 crystal. To measure the background spectrum of a blank group, before each sample measurement by Infrared Spectrometer (ENXUS-470 FT-IR), blank control with 0.9% NaCl was used in the detection of infrared absorption spectra. The parameters of measurement were the scanning range of 400 ~ 4000 cm-1, the resolution of 8 cm-1, scanning the stack up to 256 times. The data analyses were performed using OMNIC6.0 software. All the spectra were subtracted blank control, and Fourier self-deconvolution, broadband = 56.4, sensitivity enhancement factor = 2.6, in deconvolution spectrum.

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