Figure 1From: Investigations of the functional states of dendritic cells under different conditioned microenvironments by Fourier transformed infrared spectroscopy The schematic diagram of infrared spectrum of the contents of lipids and proteins in cells. Cells were adjusted to 2 × 106/ml and washed twice with 0.9% NaCl in 1000 RPM centrifugation for 6 min. The supernatant was removed by centrifugation. The cells were transferred to the CaF2 crystals at 37°C and left to stand for about 10 min. The water in the cell suspension was evaporated, until the formation of 2 ~ 3 mm film in the window. The crystals were fixed in the sample holder and covered with another CaF2 crystal. To measure the background spectrum of a blank group, before each sample measurement by Infrared Spectrometer (ENXUS-470 FT-IR), blank control with 0.9% NaCl was used in the detection of infrared absorption spectra. The parameters of measurement were the scanning range of 400 ~ 4000 cm-1, the resolution of 8 cm-1, scanning the stack up to 256 times. The data analyses were performed using OMNIC6.0 software. All the spectra were subtracted blank control, and Fourier self-deconvolution, broadband = 56.4, sensitivity enhancement factor = 2.6, in deconvolution spectrum.Back to article page