Optical images taken for the three types of cultures (A-C: monolayer; D-F: coated substrate; G-I: non-coated substrate) over three culture time points (A, D, G: 24 hours; B, E, H: 48 hours; C, F, I: 72 hours). J is the graph showing the average size of the aggregates produced on both coated and non-coated substrates over three time points; 24, 48, and 72 hours. Measurements were taken in triplicate (minimum). Error bars represent SEM. Scale bar in figures A-C is 150 μm; D-G 400 μm.