Effect of static seeding methods on the distribution of fibroblasts within human acellular dermis

Table 1 The relative seeding efficiencies after 6 hours of incubation expressed as a factor of that of the static method

Methods	Seeding efficiency /%	Factor	OD	P value
Static	42	1	0.47 ± 0.05
Cell suspension injection	20	0.5	0.23 ± 0.02	p = 0.0001
Incision	41	1	0.47 ± 0.05	p = 6
Low-pressure 20ml syringe	45	1.1	0.52 ± 0.04	p = 0.2196
Low-pressure 50ml syringe	57	1.4	0.64 ± 0.05	p = 0.0033
Ultrasonic bath	24	0.6	0.27 ± 0.04	p = 0.6505

The values are derived from the raw data from the MTT Assay (OD). The p-values are those derived from pairwise comparisons with the static method (T-test).

ISSN: 1475-925X