Table 1 Primer nucleotide sequences used in quantitative real-time RT-qPCR assays for genes described in the study
From: Different responses to mechanical injury in neonatal and adult ovine articular cartilage
Gene name | Gene symbol | Primer sequences | Ampliconsize (bp) |
|---|---|---|---|
glyceraldehyde-3-phosphate dehydrogenase | GAPDH | F:5' GTTCCACGGCACAGTCAAGG3' | 117 |
R:5' TACTCAGCACCAGCATCACCC3' | |||
mothers against DPP (Drosophila)human homologue 7 | SMAD7 | F:5' ACAACCGCAGCAGTTACCC3' | 129 |
R:5' TGTACGCCTTCTCGTAGTCAA3' | |||
peroxisome proliferator-activated receptor gamma | PPARG | F:5' GCGACATCGACCAACTGAAC3' | 274 |
R:5' ACGGAGCGAAACTGACACC3' | |||
thappin ovine molecule | TOM | F:5' CCAGGTGGTGGTGCTTCTC3' | 127 |
R:5' ACCGTTGATTGGACCCTTT3' | |||
nuclear factor-kappa B | NFκB | F:5' ACGAGGATGATGAGAATGGATG3' | 135 |
R:5' GCAGGAACACGGTTACAGGAC3' | |||
lactate dehydrogenase | LDHA | F:5' GGGACAGAATGGAATCTCAGAC3' | 296 |
R:5' TTGCCATCCAGCAGGGT3' | |||
Hypoxia-inducible factor-1α | HIF1α | F;5'-CGAAGAACTCTCAGCCACAG-3' | 174 |
R:5'-AGCTCGTGTCCT CAGATTCC-3' |