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Table 1 Main cultivation protocols of human bone marrow-derived MSC (grey: serum-free protocol)

From: Eliminating the need of serum testing using low serum culture conditions for human bone marrow-derived mesenchymal stromal cell expansion

Medium

Reference

Primary isolation demonstrated

Differentiation demonstrated

DMEM 10% FBS

Chase et al. [27], Lohmann et al. [28], Montzka et al. [23]

yes

yes

DMEM/F12 20% FBS or 20% human autologous serum or 20% human allogenic serum

Shahdadfar et al. [21]

yes

yes

DMEM 10% HPLs

Lohmann et al. [28]

yes

yes

Panserin 401 2% FBS and GF

Montzka et al. [23]

yes

yes

ESF basal medium and supplements

Mimura et. al [26]

no

yes

Stem Pro MSC SFM (Invitrogen)

Chase et al. [27]

no

yes