Cell proliferation of hMSC is not altered by cultivation with MSC-tested or non-tested serum. Cell Proliferation and cytotoxicity was monitored every 3–4 days using CellTiter-Blue Cell Viability Assay and CytoTox-ONE Homogeneous Membrane Integrity Assay. No significant differences in cell proliferation could be observed between hMSC isolated and cultivated in MSC-tested or non-tested FBS (black graph). None of the applied sera induced cell death (grey graph). Data are expressed as mean values ± SD.