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Figure 9 | BioMedical Engineering OnLine

Figure 9

From: In vitro evaluation of Panax notoginseng Rg1 released from collagen/chitosan-gelatin microsphere scaffolds for angiogenesis

Figure 9

Observation of cell attachment to and proliferation in CC-GMS scaffolds by LSCM. After 7 days of cell culture in CC-GMS scaffolds, the scaffolds and cell nuclei were stained with FITC and DAPI, respectively, and observed by LSCM. A: Control group (untreated); B: 0.1 μg/ml released Rg1; C: 1 μg/ml released Rg1; D: 10 μg/ml released Rg1; E: 50 μg/ml released Rg1; F: 100 μg/ml released Rg1; and G: 20 ng/ml VEGF. H: The relative counts of cells attached to the CC-GMS scaffold. Green and blue represent the CC-GMS scaffold and cell nuclei, respectively, photographed by a microscope at 200 ×. Significant differences compared with controls are presented (*p < 0.05).

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