Three types of dermal grafts in rats: the importance of mechanical property and structural design

Table 1 Real-time primer sequences and condition

Gene	Source^a	Primer sequences^b	Size(bp)	Annealing
CD31	NM031591.1	F 5-GGAGGTGACAGAAGGTGGGATTG-3	66	60°C
CD31	NM031591.1	R 5-GCTTGGCAGCGAAACACTAACAGG-3	66	60°C
PDGFBB	NM031524	F 5-CCCACACGTCAAACTACAGCTCCAA-3	112	60°C
PDGFBB	NM031524	R 5-GCCCAGTTCGTTTCAGTGCCACAT-3	112	60°C
α-SMA	NM031004	F 5-CCAGGGAGTGATGGTTGGAATG-3	98	60°C
α-SMA	NM031004	R 5-GCCGTGTTCTATCGGATACTTCAG-3	98	60°C
VEGF	NM031836	F 5-GGCTTTACTGCTGTACCTCCACCAT-3	161	60°C
VEGF	NM031836	R 5-CGGGGTACTCCTGGAAGATGTC-3	161	60°C
GAPDH	NM017008	F 5-GGTGGACCTCATGGCCTACAT-3	88	60°C
GAPDH	NM017008	R 5-GCCTCTCTCTTGCTCTCAGTATCCT-3	88	60°C

^aIndicates GeneBank accession numbers where applicable. ^bIndicates the sequences of forward (F) and reverse (R) primers.
Ration, respectively (n = 3).

ISSN: 1475-925X