Figure 1

Morphological evaluation of differentiated iPS cells. Differentiated iPS cells (day 19) had a polygonal shape with two nucleoli and enriched cytoplasmic granules (A,B). PAS staining demonstrated intracellular deposition of glycogen (C). Transmission electron microscopy revealed their polygonal shape and glycogen deposition in the cytoplasm (D,E). Differentiated cells partly formed duct-like structures with microvilli on the cell surface which projected into the intercellular space (F). These morphological characteristics resembled those of normal mouse hepatocytes in culture. Scale bar = 1 μm.