Figure 4
From: Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential
BMP-2 and osteopontin protein levels of MC3T3-E1 cells re-seeded on different ECMs. The ECM produced by the mechanically strained cells enhanced the relative protein expression levels of BMP-2 and OPN in cells cultured for 5 and 7 days (A/B). After 10 days of culture, the ECM had virtually no effect on the relative protein expression levels of BMP-2 and OPN (C). *P < 0.05, ** P < 0.01, between indicated groups. Quantitative results are the means ± standard error of 5 experiments.