Figure 6
From: Use of inert gas jets to measure the forces required for mechanical gene transfection
Transfection of a chicken embryo using optimized parameters. (A) Epifluorescence picture of a control chicken embryo 24 hours following treatment with the hrGFP-II 1 plasmid. (B) Epifluorescence picture of a chicken embryo 24 hours following gas treatment with the hrGFP-II 1 plasmid and helium gas at a flow rate of 1.5 L/min. The white lines show the treatment path of the 1 mm diameter capillary tube. (C) Setup of the chicken embryo for the gas flow treatment. The o-ring used to contain the treatment solution is positioned on top of the CAM. (D) The number of transfected cells was counted blindly and the results show a six-fold increase in transfection efficiency with a helium gas treatment at 2.5 L / min (n = 3 for both control and experimental, P < 0.0005). Scale bars (A,B) 1 mm.