Figure 1From: Pupillometric analysis for assessment of gene therapy in Leber Congenital Amaurosis patients Description of the off-line pupil detection algorithm steps. (a) image acquired by the pupillometer; (b) image after the thresholding; (c) image after hole filling; (d) image after small object removing; (e) original image with the pupil border enhanced in white thanks to edge detection. (f) original image with the circle (in white) which fits the pupil border.Back to article page