Figure 2

Morphological Changes during Osteogenic Stem Cell Differentiation. (a) TPEF images showing morphological changes on days 0, 2, 5, 10, 15, 20 of hMSCs undergoing ostegenic differentiation. Images are of groups exposed and unexposed to an electric field (b) Morphological changes quantified by an eccentricity ratio and graphed vs. time. Statistical differences in cell shape between the control and stimulation groups are noted by an asterisk at day 2 and 5 (p < 0.05). The means of the two groups are statistically different despite the overlap of the error bars, because the sample size of cells circled is large (n = 30).