Table 1 Primer sequences used for PCR
Genes | Primers | Size (bp) |
|---|---|---|
Aggrecan | 5-CAGAACATGCGCTCCAATGA-3' 5-CGTCATAGGTTTCGTTGGTG-3' | 371 |
Type II collagen | 5-AACCCAGAAACAACACAATCC-3' 5'-GAGGGGAGAAAAGTCCGAAC-3' | 168 |
Type X collagen | 5'-CTGAGCGATACCAAACACC-3' 5'-CCTCTCAGTGATACACCTTTAC-3' | 128 |
MMP-13 Cyclin B2 | 5'-GATAAAGACTATCCGAGAC-3' 5'-CGAACAATACGGTTACTC-3' 5'-GTTGACTATGACATGGTG-3' 5'-CAAGACAAAGTGCACGAAC-3' | 168 358 |
18S rRNA | 5'-CTACTTGGATAACTGTGGTAATTC-3' 5'-GACTCTAGATAACCTCG-3' | 168 |