Figure 4
Effect of polystyrene culture dishes (PS), Primaria® (P), and PPE:N (N) surfaces on the level of type II collagen mRNA in hypertrophic growth plate chondrocytes. Bovine hypertrophic growth plate chondrocytes were cultured for up to 20 days on the different surfaces and mRNA levels were analyzed by RT-PCR. Agarose gels show representative examples of PCR products for type II collagen (COL 2) mRNA and 18S rRNA. All values were normalized to 18S rRNA. Quantitative results are the mean ± standard error of 4 experiments.