Figure 4

MRSA infection of HUVEC under static conditions. (A) Percentage of infected HUVEC and (B) number of MRSA per HUVEC versus time. HUVEC were exposed to SYTO-9 labelled MRSA for 15, 30, or 60 minutes then analyzed by confocal microscopy to quantify the number of MRSA per HUVEC cell as described in Figure 2. Number of samples analyzed: 15 min: 3 experiments, 556 HUVEC, 2790 MRSA; 30 min: 3 experiments, 411 HUVEC, 4495 MRSA; 60 min: 3 experiments: 423 HUVEC, 4078 MRSA. Error bars are plotted as standard error of the mean (SEM).